Protocols in In Vitro Hepatocyte Research

Knowledge Source Identification
Knowledge source name Protocols in In Vitro Hepatocyte Research
Owner/Developer Springer Science+Business Media New York
Country United States of America
Languages English
Description This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.
Knowledge Source Category
Category Publication
Sub categories Book / Textbook
Knowledge Dissemination and Sharing
Dissemination channel Website, Printed
Targeted audience (specified/objective analysis) Scientists, Industry, Researchers
Users access Fee-based access
Knowledge Characterization
3Rs relevance Replacement
Purpose Fundamental studies, Toxicological and safety evaluation, Method development, Education and training, Documentation and information
Technology/Tools Stem cells, Tissue engineering, Alternative test methods (in vitro)