3D Cell Culture Methods and Protocols

Knowledge Source Identification
Knowledge source name 3D Cell Culture Methods and Protocols
Owner/Developer Springer International Publishing
Country United States of America
URL http://www.springer.com/us/book/9781607619833
Description Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.
Knowledge Source Category
Category Publication
Sub categories Book / Textbook
Knowledge Dissemination and Sharing
Dissemination channel Website, Printed
Targeted audience (specified/objective analysis) Scientists, Researchers
Users access Fee-based access
Knowledge Characterization
3Rs relevance Replacement
Purpose Method development, Education and training, Documentation and information
Technology/Tools Alternative test methods (in vitro), Tissue engineering